Activation of transcription and retrotransposition of a novel retroelement, Steamer, in neoplastic hemocytes of the mollusk Mya arenaria

Gloria Arriagada, Michael J. Metzger, Annette F. Muttray, James Sherry, Carol Reinisch, Craig Street, W. Ian Lipkin, Stephen P. Goff

Resultado de la investigación: Research - revisión exhaustivaArticle

  • 21 Citas

Resumen

Bivalve mollusks of the North Atlantic, most prominently the soft shell clam Mya arenaria, are afflicted with an epidemic transmissible disease of the circulatory system closely resembling leukemia. The disease is characterized by a dramatic expansion of blast-like cells in the hemolymph with high mitotic index. Examination of hemolymph of diseased clams revealed high levels of reverse transcriptase activity, the hallmark of retroviruses and retroelements. By deep sequencing of RNAs from hemolymph, we identified transcripts of a novel retroelement, here named Steamer. The DNA of the element is marked by long terminal repeats and encodes a single large protein with similarity to mammalian retroviral Gag-Pol proteins. Steamer mRNA levels were specifically elevated in diseased hemocytes, and high expression was correlated with disease status. DNA copy number per genome was present at enormously high levels in diseased hemocytes, indicative of extensive reverse transcription and retrotransposition. Steamer activation in M. arenaria is an example of a catastrophic induction of genetic instability that may initiate or advance the course of leukemia.

IdiomaEnglish
Páginas14175-14180
Número de páginas6
PublicaciónProceedings of the National Academy of Sciences of the United States of America
Volumen111
Número de edición39
DOI
EstadoPublished - 30 sep 2014

Huella dactilar

Mya
Hemocytes
Retroelements
Mollusca
Transcriptional Activation
Hemolymph
Bivalvia
Leukemia
DNA
gag-pol Fusion Proteins
High-Throughput Nucleotide Sequencing
Mitotic Index
Terminal Repeat Sequences
RNA-Directed DNA Polymerase
Retroviridae
Cardiovascular System
Reverse Transcription
Genome
RNA
Messenger RNA

Keywords

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    Arriagada, Gloria ; Metzger, Michael J. ; Muttray, Annette F. ; Sherry, James ; Reinisch, Carol ; Street, Craig ; Lipkin, W. Ian ; Goff, Stephen P./ Activation of transcription and retrotransposition of a novel retroelement, Steamer, in neoplastic hemocytes of the mollusk Mya arenaria. En: Proceedings of the National Academy of Sciences of the United States of America. 2014 ; Vol. 111, N.º 39. pp. 14175-14180
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    Activation of transcription and retrotransposition of a novel retroelement, Steamer, in neoplastic hemocytes of the mollusk Mya arenaria. / Arriagada, Gloria; Metzger, Michael J.; Muttray, Annette F.; Sherry, James; Reinisch, Carol; Street, Craig; Lipkin, W. Ian; Goff, Stephen P.

    En: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, N.º 39, 30.09.2014, p. 14175-14180.

    Resultado de la investigación: Research - revisión exhaustivaArticle

    TY - JOUR

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    AU - Arriagada,Gloria

    AU - Metzger,Michael J.

    AU - Muttray,Annette F.

    AU - Sherry,James

    AU - Reinisch,Carol

    AU - Street,Craig

    AU - Lipkin,W. Ian

    AU - Goff,Stephen P.

    PY - 2014/9/30

    Y1 - 2014/9/30

    N2 - Bivalve mollusks of the North Atlantic, most prominently the soft shell clam Mya arenaria, are afflicted with an epidemic transmissible disease of the circulatory system closely resembling leukemia. The disease is characterized by a dramatic expansion of blast-like cells in the hemolymph with high mitotic index. Examination of hemolymph of diseased clams revealed high levels of reverse transcriptase activity, the hallmark of retroviruses and retroelements. By deep sequencing of RNAs from hemolymph, we identified transcripts of a novel retroelement, here named Steamer. The DNA of the element is marked by long terminal repeats and encodes a single large protein with similarity to mammalian retroviral Gag-Pol proteins. Steamer mRNA levels were specifically elevated in diseased hemocytes, and high expression was correlated with disease status. DNA copy number per genome was present at enormously high levels in diseased hemocytes, indicative of extensive reverse transcription and retrotransposition. Steamer activation in M. arenaria is an example of a catastrophic induction of genetic instability that may initiate or advance the course of leukemia.

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    KW - Disseminated neoplasia

    KW - Hemic neoplasia

    KW - Integration

    KW - Mobile genetic element

    KW - Retrotransposon

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