Adoptive transfer of autoimmune splenic dendritic cells to lupus-prone mice triggers a B lymphocyte humoral response

Daniela Sauma, Natalia Crisóstomo, Camila Fuentes, María Alejandra Gleisner, Yessia Hidalgo, María José Fuenzalida, Mario Rosemblatt, María Rosa Bono

Resultado de la investigación: Contribución a la publicaciónArticle

Resumen

Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by increased autoantibody production that leads to multiple tissue injuries. Dendritic cells (DCs) are important orchestrators of immune responses and key components in fine-tuning the balance between tolerance and immunity. However, their role in autoimmune disorders such as SLE remains uncertain. We analyzed the contribution of DCs in triggering SLE by adoptively transferring splenic DCs from aged autoimmune [NZB×NZW]F1 (BWF1) mice to young healthy BWF1 mice. We observed that the transfer of DCs from autoimmune mice to pre-autoimmune mice induced high autoantibody titers in the serum of recipient mice. Moreover, autoimmune DCs from aged BWF1 mice were crucial for the expansion and differentiation of plasmablasts and CD5+ B cells or B1-like cells in the peripheral blood, and spleen of recipient BWF1 mice, a phenomenon that is observed in autoimmune BWF1 mice. On the other hand, DCs from aged BWF1 mice participated in the expansion and differentiation of DCs and IFN-γ-producing T cells. These results reveal that DCs from autoimmune BWF1 mice exhibit functional and phenotypic characteristics that allow them to trigger B cell hyperactivation, as well as DC and T cell expansion and differentiation, thereby promoting an exacerbated humoral response in lupus-prone mice.

Idioma originalEnglish
Páginas (desde - hasta)957-968
Número de páginas12
PublicaciónImmunologic Research
Volumen65
Número de edición4
Identificadores de objetos digitales
EstadoPublished - 1 ago 2017

Huella dactilar

Adoptive Transfer
Dendritic Cells
B-Lymphocytes
Systemic Lupus Erythematosus
Autoantibodies
T-Lymphocytes
Multiple Trauma
Autoimmune Diseases
Cell Differentiation
Immunity
Spleen
Serum

Keywords

    ASJC Scopus subject areas

    • Immunology

    Citar esto

    Sauma, D., Crisóstomo, N., Fuentes, C., Gleisner, M. A., Hidalgo, Y., Fuenzalida, M. J., ... Bono, M. R. (2017). Adoptive transfer of autoimmune splenic dendritic cells to lupus-prone mice triggers a B lymphocyte humoral response. Immunologic Research, 65(4), 957-968. DOI: 10.1007/s12026-017-8936-9

    Sauma, Daniela; Crisóstomo, Natalia; Fuentes, Camila; Gleisner, María Alejandra; Hidalgo, Yessia; Fuenzalida, María José; Rosemblatt, Mario; Bono, María Rosa / Adoptive transfer of autoimmune splenic dendritic cells to lupus-prone mice triggers a B lymphocyte humoral response.

    En: Immunologic Research, Vol. 65, N.º 4, 01.08.2017, p. 957-968.

    Resultado de la investigación: Contribución a la publicaciónArticle

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    title = "Adoptive transfer of autoimmune splenic dendritic cells to lupus-prone mice triggers a B lymphocyte humoral response",
    abstract = "Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by increased autoantibody production that leads to multiple tissue injuries. Dendritic cells (DCs) are important orchestrators of immune responses and key components in fine-tuning the balance between tolerance and immunity. However, their role in autoimmune disorders such as SLE remains uncertain. We analyzed the contribution of DCs in triggering SLE by adoptively transferring splenic DCs from aged autoimmune [NZB×NZW]F1 (BWF1) mice to young healthy BWF1 mice. We observed that the transfer of DCs from autoimmune mice to pre-autoimmune mice induced high autoantibody titers in the serum of recipient mice. Moreover, autoimmune DCs from aged BWF1 mice were crucial for the expansion and differentiation of plasmablasts and CD5+ B cells or B1-like cells in the peripheral blood, and spleen of recipient BWF1 mice, a phenomenon that is observed in autoimmune BWF1 mice. On the other hand, DCs from aged BWF1 mice participated in the expansion and differentiation of DCs and IFN-γ-producing T cells. These results reveal that DCs from autoimmune BWF1 mice exhibit functional and phenotypic characteristics that allow them to trigger B cell hyperactivation, as well as DC and T cell expansion and differentiation, thereby promoting an exacerbated humoral response in lupus-prone mice.",
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    Sauma, D, Crisóstomo, N, Fuentes, C, Gleisner, MA, Hidalgo, Y, Fuenzalida, MJ, Rosemblatt, M & Bono, MR 2017, 'Adoptive transfer of autoimmune splenic dendritic cells to lupus-prone mice triggers a B lymphocyte humoral response' Immunologic Research, vol. 65, n.º 4, pp. 957-968. DOI: 10.1007/s12026-017-8936-9

    Adoptive transfer of autoimmune splenic dendritic cells to lupus-prone mice triggers a B lymphocyte humoral response. / Sauma, Daniela; Crisóstomo, Natalia; Fuentes, Camila; Gleisner, María Alejandra; Hidalgo, Yessia; Fuenzalida, María José; Rosemblatt, Mario; Bono, María Rosa.

    En: Immunologic Research, Vol. 65, N.º 4, 01.08.2017, p. 957-968.

    Resultado de la investigación: Contribución a la publicaciónArticle

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    AU - Sauma,Daniela

    AU - Crisóstomo,Natalia

    AU - Fuentes,Camila

    AU - Gleisner,María Alejandra

    AU - Hidalgo,Yessia

    AU - Fuenzalida,María José

    AU - Rosemblatt,Mario

    AU - Bono,María Rosa

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    N2 - Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by increased autoantibody production that leads to multiple tissue injuries. Dendritic cells (DCs) are important orchestrators of immune responses and key components in fine-tuning the balance between tolerance and immunity. However, their role in autoimmune disorders such as SLE remains uncertain. We analyzed the contribution of DCs in triggering SLE by adoptively transferring splenic DCs from aged autoimmune [NZB×NZW]F1 (BWF1) mice to young healthy BWF1 mice. We observed that the transfer of DCs from autoimmune mice to pre-autoimmune mice induced high autoantibody titers in the serum of recipient mice. Moreover, autoimmune DCs from aged BWF1 mice were crucial for the expansion and differentiation of plasmablasts and CD5+ B cells or B1-like cells in the peripheral blood, and spleen of recipient BWF1 mice, a phenomenon that is observed in autoimmune BWF1 mice. On the other hand, DCs from aged BWF1 mice participated in the expansion and differentiation of DCs and IFN-γ-producing T cells. These results reveal that DCs from autoimmune BWF1 mice exhibit functional and phenotypic characteristics that allow them to trigger B cell hyperactivation, as well as DC and T cell expansion and differentiation, thereby promoting an exacerbated humoral response in lupus-prone mice.

    AB - Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by increased autoantibody production that leads to multiple tissue injuries. Dendritic cells (DCs) are important orchestrators of immune responses and key components in fine-tuning the balance between tolerance and immunity. However, their role in autoimmune disorders such as SLE remains uncertain. We analyzed the contribution of DCs in triggering SLE by adoptively transferring splenic DCs from aged autoimmune [NZB×NZW]F1 (BWF1) mice to young healthy BWF1 mice. We observed that the transfer of DCs from autoimmune mice to pre-autoimmune mice induced high autoantibody titers in the serum of recipient mice. Moreover, autoimmune DCs from aged BWF1 mice were crucial for the expansion and differentiation of plasmablasts and CD5+ B cells or B1-like cells in the peripheral blood, and spleen of recipient BWF1 mice, a phenomenon that is observed in autoimmune BWF1 mice. On the other hand, DCs from aged BWF1 mice participated in the expansion and differentiation of DCs and IFN-γ-producing T cells. These results reveal that DCs from autoimmune BWF1 mice exhibit functional and phenotypic characteristics that allow them to trigger B cell hyperactivation, as well as DC and T cell expansion and differentiation, thereby promoting an exacerbated humoral response in lupus-prone mice.

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    KW - B lymphocytes

    KW - Dendritic cells

    KW - Humoral response

    KW - Systemic lupus erythematosus

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    Sauma D, Crisóstomo N, Fuentes C, Gleisner MA, Hidalgo Y, Fuenzalida MJ y otros. Adoptive transfer of autoimmune splenic dendritic cells to lupus-prone mice triggers a B lymphocyte humoral response. Immunologic Research. 2017 ago 1;65(4):957-968. Disponible desde, DOI: 10.1007/s12026-017-8936-9