Antiplatelet effect of differentially charged PEGylated lipid-polymer nanoparticles

Eduardo Fuentes, Basit Yameen, Soung Jae Bong, Carolina Salvador-Morales, Ivan Palomo, Cristian Vilos

Resultado de la investigación: Research - revisión exhaustivaArticle

  • 1 Citas

Resumen

PEGylated nanoparticles have been extensively investigated in different platforms for drug delivery. However, the physiological effects related to platelet activation, and the potential procoagulant activity which could lead to thrombosis and further cardiovascular diseases have not been widely examined. In this work, we studied the effect of differentially charged PEGylated lipid-polymer nanoparticles in the human platelet aggregation and activation by light transmission aggregometry and flow cytometry. PEGylated nanoparticles inhibited the platelet aggregation with a dose dependency (350, 700, and 1400 μg/mL) in both ADP- and collagen-induced platelet aggregation, and P-selectin expression. Charged nanoparticles (anionic and cationic) presented higher inhibitions of the platelet aggregation compared to neutral nanoparticles, and particularly the cationic particles generated a slightly higher effect. The obtained results demonstrated the safety of the differentially charged PEGylated lipid-polymer nanoparticles, and their ability to inhibit the aggregation and activation of human platelets stimulated by two classic platelet activators.

IdiomaEnglish
Páginas1089-1094
Número de páginas6
PublicaciónNanomedicine: Nanotechnology, Biology, and Medicine
Volumen13
Número de edición3
DOI
EstadoPublished - 1 abr 2017

Huella dactilar

Platelets
Polymers
Nanoparticles
Lipids
Agglomeration
Platelet Aggregation
Platelet Activation
Chemical activation
P-Selectin
Adenosine Diphosphate
Flow Cytometry
Thrombosis
Cardiovascular Diseases
Collagen
Blood Platelets
Safety
Light
Pharmaceutical Preparations
Flow cytometry
Light transmission

Keywords

    ASJC Scopus subject areas

    • Medicine (miscellaneous)
    • Bioengineering
    • Biomedical Engineering
    • Molecular Medicine
    • Materials Science(all)
    • Pharmaceutical Science

    Citar esto

    Fuentes, Eduardo ; Yameen, Basit ; Bong, Soung Jae ; Salvador-Morales, Carolina ; Palomo, Ivan ; Vilos, Cristian. / Antiplatelet effect of differentially charged PEGylated lipid-polymer nanoparticles. En: Nanomedicine: Nanotechnology, Biology, and Medicine. 2017 ; Vol. 13, N.º 3. pp. 1089-1094
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    Antiplatelet effect of differentially charged PEGylated lipid-polymer nanoparticles. / Fuentes, Eduardo; Yameen, Basit; Bong, Soung Jae; Salvador-Morales, Carolina; Palomo, Ivan; Vilos, Cristian.

    En: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 13, N.º 3, 01.04.2017, p. 1089-1094.

    Resultado de la investigación: Research - revisión exhaustivaArticle

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    Fuentes E, Yameen B, Bong SJ, Salvador-Morales C, Palomo I, Vilos C. Antiplatelet effect of differentially charged PEGylated lipid-polymer nanoparticles. Nanomedicine: Nanotechnology, Biology, and Medicine. 2017 abr 1;13(3):1089-1094. Disponible desde, DOI: 10.1016/j.nano.2016.10.010