BZIP17 and bZIP60 Regulate the Expression of BiP3 and Other Salt Stress Responsive Genes in an UPR-Independent Manner in Arabidopsis thaliana

Carlos Henriquez-Valencia, Adrian A. Moreno, Omar Sandoval-Ibañez, Irina Mitina, Francisca Blanco-Herrera, Nicolas Cifuentes-Esquivel, Ariel Orellana

Resultado de la investigación: Contribución a la publicaciónArticle

  • 11 Citas

Resumen

Plants can be severely affected by salt stress. Since these are sessile organisms, they have developed different cellular responses to cope with this problem. Recently, it has been described that bZIP17 and bZIP60, two ER-located transcription factors, are involved in the cellular response to salt stress. On the other hand, bZIP60 is also involved in the unfolded protein response (UPR), a signaling pathway that up-regulates the expression of ER-chaperones. Coincidentally, salt stress produces the up-regulation of BiP, one of the main chaperones located in this organelle. Then, it has been proposed that UPR is associated to salt stress. Here, by using insertional mutant plants on bZIP17 and bZIP60, we show that bZIP17 regulate the accumulation of the transcript for the chaperone BiP3 under salt stress conditions, but does not lead to the accumulation of UPR-responding genes such as the chaperones Calnexin, Calreticulin, and PDIL under salt treatments. In contrast, DTT, a known inducer of UPR, leads to the up-regulation of all these chaperones. On the other hand, we found that bZIP60 regulates the expression of some bZIP17 target genes under conditions were splicing of bZIP60 does not occur, suggesting that the spliced and unspliced forms of bZIP60 play different roles in the physiological response of the plant. Our results indicate that the ER-located transcription factors bZIP17 and bZIP60 play a role in salt stress but this response goes through a signaling pathway that is different to that triggered by the unfolded protein response. J. Cell. Biochem. 116: 1638-1645, 2015.

Idioma originalEnglish
Páginas (desde - hasta)1638-1645
Número de páginas8
PublicaciónJournal of Cellular Biochemistry
Volumen116
Número de edición8
Identificadores de objetos digitales
EstadoPublished - 1 ago 2015

Huella dactilar

Unfolded Protein Response
Arabidopsis
Salts
Genes
Up-Regulation
Transcription Factors
Calnexin
Calreticulin
Organelles

Keywords

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Citar esto

    Henriquez-Valencia, Carlos; Moreno, Adrian A.; Sandoval-Ibañez, Omar; Mitina, Irina; Blanco-Herrera, Francisca; Cifuentes-Esquivel, Nicolas; Orellana, Ariel / BZIP17 and bZIP60 Regulate the Expression of BiP3 and Other Salt Stress Responsive Genes in an UPR-Independent Manner in Arabidopsis thaliana.

    En: Journal of Cellular Biochemistry, Vol. 116, N.º 8, 01.08.2015, p. 1638-1645.

    Resultado de la investigación: Contribución a la publicaciónArticle

    @article{3636945c3993431ab857e0a193f90aff,
    title = "BZIP17 and bZIP60 Regulate the Expression of BiP3 and Other Salt Stress Responsive Genes in an UPR-Independent Manner in Arabidopsis thaliana",
    abstract = "Plants can be severely affected by salt stress. Since these are sessile organisms, they have developed different cellular responses to cope with this problem. Recently, it has been described that bZIP17 and bZIP60, two ER-located transcription factors, are involved in the cellular response to salt stress. On the other hand, bZIP60 is also involved in the unfolded protein response (UPR), a signaling pathway that up-regulates the expression of ER-chaperones. Coincidentally, salt stress produces the up-regulation of BiP, one of the main chaperones located in this organelle. Then, it has been proposed that UPR is associated to salt stress. Here, by using insertional mutant plants on bZIP17 and bZIP60, we show that bZIP17 regulate the accumulation of the transcript for the chaperone BiP3 under salt stress conditions, but does not lead to the accumulation of UPR-responding genes such as the chaperones Calnexin, Calreticulin, and PDIL under salt treatments. In contrast, DTT, a known inducer of UPR, leads to the up-regulation of all these chaperones. On the other hand, we found that bZIP60 regulates the expression of some bZIP17 target genes under conditions were splicing of bZIP60 does not occur, suggesting that the spliced and unspliced forms of bZIP60 play different roles in the physiological response of the plant. Our results indicate that the ER-located transcription factors bZIP17 and bZIP60 play a role in salt stress but this response goes through a signaling pathway that is different to that triggered by the unfolded protein response. J. Cell. Biochem. 116: 1638-1645, 2015.",
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    author = "Carlos Henriquez-Valencia and Moreno, {Adrian A.} and Omar Sandoval-Ibañez and Irina Mitina and Francisca Blanco-Herrera and Nicolas Cifuentes-Esquivel and Ariel Orellana",
    year = "2015",
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    BZIP17 and bZIP60 Regulate the Expression of BiP3 and Other Salt Stress Responsive Genes in an UPR-Independent Manner in Arabidopsis thaliana. / Henriquez-Valencia, Carlos; Moreno, Adrian A.; Sandoval-Ibañez, Omar; Mitina, Irina; Blanco-Herrera, Francisca; Cifuentes-Esquivel, Nicolas; Orellana, Ariel.

    En: Journal of Cellular Biochemistry, Vol. 116, N.º 8, 01.08.2015, p. 1638-1645.

    Resultado de la investigación: Contribución a la publicaciónArticle

    TY - JOUR

    T1 - BZIP17 and bZIP60 Regulate the Expression of BiP3 and Other Salt Stress Responsive Genes in an UPR-Independent Manner in Arabidopsis thaliana

    AU - Henriquez-Valencia,Carlos

    AU - Moreno,Adrian A.

    AU - Sandoval-Ibañez,Omar

    AU - Mitina,Irina

    AU - Blanco-Herrera,Francisca

    AU - Cifuentes-Esquivel,Nicolas

    AU - Orellana,Ariel

    PY - 2015/8/1

    Y1 - 2015/8/1

    N2 - Plants can be severely affected by salt stress. Since these are sessile organisms, they have developed different cellular responses to cope with this problem. Recently, it has been described that bZIP17 and bZIP60, two ER-located transcription factors, are involved in the cellular response to salt stress. On the other hand, bZIP60 is also involved in the unfolded protein response (UPR), a signaling pathway that up-regulates the expression of ER-chaperones. Coincidentally, salt stress produces the up-regulation of BiP, one of the main chaperones located in this organelle. Then, it has been proposed that UPR is associated to salt stress. Here, by using insertional mutant plants on bZIP17 and bZIP60, we show that bZIP17 regulate the accumulation of the transcript for the chaperone BiP3 under salt stress conditions, but does not lead to the accumulation of UPR-responding genes such as the chaperones Calnexin, Calreticulin, and PDIL under salt treatments. In contrast, DTT, a known inducer of UPR, leads to the up-regulation of all these chaperones. On the other hand, we found that bZIP60 regulates the expression of some bZIP17 target genes under conditions were splicing of bZIP60 does not occur, suggesting that the spliced and unspliced forms of bZIP60 play different roles in the physiological response of the plant. Our results indicate that the ER-located transcription factors bZIP17 and bZIP60 play a role in salt stress but this response goes through a signaling pathway that is different to that triggered by the unfolded protein response. J. Cell. Biochem. 116: 1638-1645, 2015.

    AB - Plants can be severely affected by salt stress. Since these are sessile organisms, they have developed different cellular responses to cope with this problem. Recently, it has been described that bZIP17 and bZIP60, two ER-located transcription factors, are involved in the cellular response to salt stress. On the other hand, bZIP60 is also involved in the unfolded protein response (UPR), a signaling pathway that up-regulates the expression of ER-chaperones. Coincidentally, salt stress produces the up-regulation of BiP, one of the main chaperones located in this organelle. Then, it has been proposed that UPR is associated to salt stress. Here, by using insertional mutant plants on bZIP17 and bZIP60, we show that bZIP17 regulate the accumulation of the transcript for the chaperone BiP3 under salt stress conditions, but does not lead to the accumulation of UPR-responding genes such as the chaperones Calnexin, Calreticulin, and PDIL under salt treatments. In contrast, DTT, a known inducer of UPR, leads to the up-regulation of all these chaperones. On the other hand, we found that bZIP60 regulates the expression of some bZIP17 target genes under conditions were splicing of bZIP60 does not occur, suggesting that the spliced and unspliced forms of bZIP60 play different roles in the physiological response of the plant. Our results indicate that the ER-located transcription factors bZIP17 and bZIP60 play a role in salt stress but this response goes through a signaling pathway that is different to that triggered by the unfolded protein response. J. Cell. Biochem. 116: 1638-1645, 2015.

    KW - Arabidopsis thaliana

    KW - bZIP17

    KW - bZIP60

    KW - SALINITY STRESS

    KW - TRANSCRIPTION FACTOR

    KW - UPR

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    U2 - 10.1002/jcb.25121

    DO - 10.1002/jcb.25121

    M3 - Article

    VL - 116

    SP - 1638

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    JO - Journal of Cellular Biochemistry

    T2 - Journal of Cellular Biochemistry

    JF - Journal of Cellular Biochemistry

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    ER -

    Henriquez-Valencia C, Moreno AA, Sandoval-Ibañez O, Mitina I, Blanco-Herrera F, Cifuentes-Esquivel N y otros. BZIP17 and bZIP60 Regulate the Expression of BiP3 and Other Salt Stress Responsive Genes in an UPR-Independent Manner in Arabidopsis thaliana. Journal of Cellular Biochemistry. 2015 ago 1;116(8):1638-1645. Disponible desde, DOI: 10.1002/jcb.25121