Comparative genomics analysis of a new Exiguobacterium strain from salar de huasco reveals a repertoire of stress-related genes and arsenic resistance

Juan Castro-Severyn, Francisco Remonsellez, Sandro L. Valenzuela, Cesar Salinas, Jonathan Fortt, Pablo Aguilar, Coral Pardo-Esté, Cristina Dorador, Raquel Quatrini, Franck Molina, Daniel Aguayo, Eduardo Castro-Nallar, Claudia P. Saavedra

Resultado de la investigación: Contribución a la publicaciónArticle

Resumen

The Atacama Desert hosts diverse ecosystems including salt flats and shallow Andean lakes. Several heavy metals are found in the Atacama Desert, and microorganisms growing in this environment show varying levels of resistance/tolerance to copper, tellurium, and arsenic, among others. Herein, we report the genome sequence and comparative genomic analysis of a new Exiguobacterium strain, sp. SH31, isolated from an altiplanic shallow athalassohaline lake. Exiguobacterium sp. SH31 belongs to the phylogenetic Group II and its closest relative is Exiguobacterium sp. S17, isolated from the Argentinian Altiplano (95% average nucleotide identity). Strain SH31 encodes a wide repertoire of proteins required for cadmium, copper, mercury, tellurium, chromium, and arsenic resistance. Of the 34 Exiguobacterium genomes that were inspected, only isolates SH31 and S17 encode the arsenic efflux pump Acr3. Strain SH31 was able to grow in up to 10 mM arsenite and 100 mM arsenate, indicating that it is arsenic resistant. Further, expression of the ars operon and acr3 was strongly induced in response to both toxics, suggesting that the arsenic efflux pump Acr3 mediates arsenic resistance in Exiguobacterium sp. SH31.

Idioma originalEnglish
Número de artículo456
PublicaciónFrontiers in Microbiology
Volumen8
Número de ediciónMAR
Identificadores de objetos digitales
EstadoPublished - 21 mar 2017

Huella dactilar

Arsenic
Genomics
Genes
Tellurium
Lakes
Copper
Genome
Poisons
Chromium
Operon
Heavy Metals
Mercury
Cadmium
Ecosystem
Nucleotides
Salts
Proteins

Keywords

    ASJC Scopus subject areas

    • Microbiology
    • Microbiology (medical)

    Citar esto

    Castro-Severyn, Juan; Remonsellez, Francisco; Valenzuela, Sandro L.; Salinas, Cesar; Fortt, Jonathan; Aguilar, Pablo; Pardo-Esté, Coral; Dorador, Cristina; Quatrini, Raquel; Molina, Franck; Aguayo, Daniel; Castro-Nallar, Eduardo; Saavedra, Claudia P. / Comparative genomics analysis of a new Exiguobacterium strain from salar de huasco reveals a repertoire of stress-related genes and arsenic resistance.

    En: Frontiers in Microbiology, Vol. 8, N.º MAR, 456, 21.03.2017.

    Resultado de la investigación: Contribución a la publicaciónArticle

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    abstract = "The Atacama Desert hosts diverse ecosystems including salt flats and shallow Andean lakes. Several heavy metals are found in the Atacama Desert, and microorganisms growing in this environment show varying levels of resistance/tolerance to copper, tellurium, and arsenic, among others. Herein, we report the genome sequence and comparative genomic analysis of a new Exiguobacterium strain, sp. SH31, isolated from an altiplanic shallow athalassohaline lake. Exiguobacterium sp. SH31 belongs to the phylogenetic Group II and its closest relative is Exiguobacterium sp. S17, isolated from the Argentinian Altiplano (95% average nucleotide identity). Strain SH31 encodes a wide repertoire of proteins required for cadmium, copper, mercury, tellurium, chromium, and arsenic resistance. Of the 34 Exiguobacterium genomes that were inspected, only isolates SH31 and S17 encode the arsenic efflux pump Acr3. Strain SH31 was able to grow in up to 10 mM arsenite and 100 mM arsenate, indicating that it is arsenic resistant. Further, expression of the ars operon and acr3 was strongly induced in response to both toxics, suggesting that the arsenic efflux pump Acr3 mediates arsenic resistance in Exiguobacterium sp. SH31.",
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    Castro-Severyn, J, Remonsellez, F, Valenzuela, SL, Salinas, C, Fortt, J, Aguilar, P, Pardo-Esté, C, Dorador, C, Quatrini, R, Molina, F, Aguayo, D, Castro-Nallar, E & Saavedra, CP 2017, 'Comparative genomics analysis of a new Exiguobacterium strain from salar de huasco reveals a repertoire of stress-related genes and arsenic resistance' Frontiers in Microbiology, vol. 8, n.º MAR, 456. DOI: 10.3389/fmicb.2017.00456

    Comparative genomics analysis of a new Exiguobacterium strain from salar de huasco reveals a repertoire of stress-related genes and arsenic resistance. / Castro-Severyn, Juan; Remonsellez, Francisco; Valenzuela, Sandro L.; Salinas, Cesar; Fortt, Jonathan; Aguilar, Pablo; Pardo-Esté, Coral; Dorador, Cristina; Quatrini, Raquel; Molina, Franck; Aguayo, Daniel; Castro-Nallar, Eduardo; Saavedra, Claudia P.

    En: Frontiers in Microbiology, Vol. 8, N.º MAR, 456, 21.03.2017.

    Resultado de la investigación: Contribución a la publicaciónArticle

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    AU - Castro-Severyn,Juan

    AU - Remonsellez,Francisco

    AU - Valenzuela,Sandro L.

    AU - Salinas,Cesar

    AU - Fortt,Jonathan

    AU - Aguilar,Pablo

    AU - Pardo-Esté,Coral

    AU - Dorador,Cristina

    AU - Quatrini,Raquel

    AU - Molina,Franck

    AU - Aguayo,Daniel

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    AU - Saavedra,Claudia P.

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    KW - Comparative genomics

    KW - Exiguobacterium

    KW - Polyextremophile

    KW - Stress

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    Castro-Severyn J, Remonsellez F, Valenzuela SL, Salinas C, Fortt J, Aguilar P y otros. Comparative genomics analysis of a new Exiguobacterium strain from salar de huasco reveals a repertoire of stress-related genes and arsenic resistance. Frontiers in Microbiology. 2017 mar 21;8(MAR). 456. Disponible desde, DOI: 10.3389/fmicb.2017.00456