Consecutive emamectin benzoate and deltamethrin treatments affect the expressions and activities of detoxification enzymes in the rainbow trout (Oncorhynchus mykiss)

Juan Guillermo Cárcamo, Marcelo N. Aguilar, Constanza F. Carreño, Tamara Vera, Luis Arias-Darraz, Jaime E. Figueroa, Alex P. Romero, Marco Alvarez, Alejandro J. Yañez

Resultado de la investigación: Research - revisión exhaustivaArticle

  • 1 Citas

Resumen

Rainbow trout (Oncorhynchus mykiss) subjected to three consecutive, alternating treatments with emamectin benzoate (EMB) and deltamethrin (DM) during outbreaks of Caligus rogercresseyi in a farm located in southern Chile (Hornopiren, Chiloé), were studied to determine the effects of these treatments on the protein and enzymatic activity levels of cytochrome P450 1A (CYP1A), flavin-containing monooxygenase (FMO) and glutathione S-transferase (GST) in different tissues. Consecutive and alternating EMB/DM treatments resulted in a 10-fold increase and 3-fold decrease of CYP1A protein levels in the intestine and gills, respectively. Notably, CYP1A activity levels decreased in most of the analyzed tissues. FMO protein and activity levels markedly increased in the kidney and the intestine. GST was up-regulated in all tissues, either as protein or enzyme activity. When comparing consecutive EMB/DM treatments against previous studies of EMB treatment alone, CYP1A activity levels were similarly diminished, except in muscle. Likewise, FMO activity levels were increased in most of the analyzed tissues, particularly in the muscle, kidney, and intestine. The increases observed for GST were essentially unchanged between consecutive EMB/DM and EMB only treatments. These results indicate that consecutive EMB/DM treatments in rainbow trout induce the expression and activity of FMO and GST enzymes and decrease CYP1A activity. These altered activities of detoxification enzymes could generate imbalances in metabolic processes, synthesis, degradation of hormones and complications associated with drug interactions. It is especially important when analyzing possible effects of consecutive antiparasitic treatments on withholding periods and salmon farming yields.

IdiomaEnglish
Páginas129-137
Número de páginas9
PublicaciónComparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Volumen191
DOI
EstadoPublished - 1 ene 2017

Huella dactilar

Oncorhynchus mykiss
Enzymes
decamethrin
emamectin benzoate
Detoxification
Therapeutics
Cytochrome P-450 Enzyme System
dimethylaniline monooxygenase (N-oxide forming)
Glutathione Transferase
Proteins
Tissue
Intestines
Kidney
Muscles
Muscle
Antiparasitic Agents
Withholding Treatment
Chile
Salmon
Agriculture

Keywords

    ASJC Scopus subject areas

    • Physiology
    • Biochemistry
    • Toxicology
    • Cell Biology
    • Health, Toxicology and Mutagenesis
    • Medicine(all)

    Citar esto

    Cárcamo, Juan Guillermo ; Aguilar, Marcelo N. ; Carreño, Constanza F. ; Vera, Tamara ; Arias-Darraz, Luis ; Figueroa, Jaime E. ; Romero, Alex P. ; Alvarez, Marco ; Yañez, Alejandro J./ Consecutive emamectin benzoate and deltamethrin treatments affect the expressions and activities of detoxification enzymes in the rainbow trout (Oncorhynchus mykiss). En: Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology. 2017 ; Vol. 191. pp. 129-137
    @article{4f1f09b405044266961e63d67c2f791b,
    title = "Consecutive emamectin benzoate and deltamethrin treatments affect the expressions and activities of detoxification enzymes in the rainbow trout (Oncorhynchus mykiss)",
    abstract = "Rainbow trout (Oncorhynchus mykiss) subjected to three consecutive, alternating treatments with emamectin benzoate (EMB) and deltamethrin (DM) during outbreaks of Caligus rogercresseyi in a farm located in southern Chile (Hornopiren, Chiloé), were studied to determine the effects of these treatments on the protein and enzymatic activity levels of cytochrome P450 1A (CYP1A), flavin-containing monooxygenase (FMO) and glutathione S-transferase (GST) in different tissues. Consecutive and alternating EMB/DM treatments resulted in a 10-fold increase and 3-fold decrease of CYP1A protein levels in the intestine and gills, respectively. Notably, CYP1A activity levels decreased in most of the analyzed tissues. FMO protein and activity levels markedly increased in the kidney and the intestine. GST was up-regulated in all tissues, either as protein or enzyme activity. When comparing consecutive EMB/DM treatments against previous studies of EMB treatment alone, CYP1A activity levels were similarly diminished, except in muscle. Likewise, FMO activity levels were increased in most of the analyzed tissues, particularly in the muscle, kidney, and intestine. The increases observed for GST were essentially unchanged between consecutive EMB/DM and EMB only treatments. These results indicate that consecutive EMB/DM treatments in rainbow trout induce the expression and activity of FMO and GST enzymes and decrease CYP1A activity. These altered activities of detoxification enzymes could generate imbalances in metabolic processes, synthesis, degradation of hormones and complications associated with drug interactions. It is especially important when analyzing possible effects of consecutive antiparasitic treatments on withholding periods and salmon farming yields.",
    keywords = "Cytochrome P450, Deltamethrin, Drug detoxification, Emamectin benzoate, Flavin-monooxygenase, Glutathione S-transferase",
    author = "Cárcamo, {Juan Guillermo} and Aguilar, {Marcelo N.} and Carreño, {Constanza F.} and Tamara Vera and Luis Arias-Darraz and Figueroa, {Jaime E.} and Romero, {Alex P.} and Marco Alvarez and Yañez, {Alejandro J.}",
    year = "2017",
    month = "1",
    doi = "10.1016/j.cbpc.2016.10.004",
    volume = "191",
    pages = "129--137",
    journal = "Comparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology",
    issn = "0742-8413",
    publisher = "Elsevier Inc.",

    }

    Consecutive emamectin benzoate and deltamethrin treatments affect the expressions and activities of detoxification enzymes in the rainbow trout (Oncorhynchus mykiss). / Cárcamo, Juan Guillermo; Aguilar, Marcelo N.; Carreño, Constanza F.; Vera, Tamara; Arias-Darraz, Luis; Figueroa, Jaime E.; Romero, Alex P.; Alvarez, Marco; Yañez, Alejandro J.

    En: Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, Vol. 191, 01.01.2017, p. 129-137.

    Resultado de la investigación: Research - revisión exhaustivaArticle

    TY - JOUR

    T1 - Consecutive emamectin benzoate and deltamethrin treatments affect the expressions and activities of detoxification enzymes in the rainbow trout (Oncorhynchus mykiss)

    AU - Cárcamo,Juan Guillermo

    AU - Aguilar,Marcelo N.

    AU - Carreño,Constanza F.

    AU - Vera,Tamara

    AU - Arias-Darraz,Luis

    AU - Figueroa,Jaime E.

    AU - Romero,Alex P.

    AU - Alvarez,Marco

    AU - Yañez,Alejandro J.

    PY - 2017/1/1

    Y1 - 2017/1/1

    N2 - Rainbow trout (Oncorhynchus mykiss) subjected to three consecutive, alternating treatments with emamectin benzoate (EMB) and deltamethrin (DM) during outbreaks of Caligus rogercresseyi in a farm located in southern Chile (Hornopiren, Chiloé), were studied to determine the effects of these treatments on the protein and enzymatic activity levels of cytochrome P450 1A (CYP1A), flavin-containing monooxygenase (FMO) and glutathione S-transferase (GST) in different tissues. Consecutive and alternating EMB/DM treatments resulted in a 10-fold increase and 3-fold decrease of CYP1A protein levels in the intestine and gills, respectively. Notably, CYP1A activity levels decreased in most of the analyzed tissues. FMO protein and activity levels markedly increased in the kidney and the intestine. GST was up-regulated in all tissues, either as protein or enzyme activity. When comparing consecutive EMB/DM treatments against previous studies of EMB treatment alone, CYP1A activity levels were similarly diminished, except in muscle. Likewise, FMO activity levels were increased in most of the analyzed tissues, particularly in the muscle, kidney, and intestine. The increases observed for GST were essentially unchanged between consecutive EMB/DM and EMB only treatments. These results indicate that consecutive EMB/DM treatments in rainbow trout induce the expression and activity of FMO and GST enzymes and decrease CYP1A activity. These altered activities of detoxification enzymes could generate imbalances in metabolic processes, synthesis, degradation of hormones and complications associated with drug interactions. It is especially important when analyzing possible effects of consecutive antiparasitic treatments on withholding periods and salmon farming yields.

    AB - Rainbow trout (Oncorhynchus mykiss) subjected to three consecutive, alternating treatments with emamectin benzoate (EMB) and deltamethrin (DM) during outbreaks of Caligus rogercresseyi in a farm located in southern Chile (Hornopiren, Chiloé), were studied to determine the effects of these treatments on the protein and enzymatic activity levels of cytochrome P450 1A (CYP1A), flavin-containing monooxygenase (FMO) and glutathione S-transferase (GST) in different tissues. Consecutive and alternating EMB/DM treatments resulted in a 10-fold increase and 3-fold decrease of CYP1A protein levels in the intestine and gills, respectively. Notably, CYP1A activity levels decreased in most of the analyzed tissues. FMO protein and activity levels markedly increased in the kidney and the intestine. GST was up-regulated in all tissues, either as protein or enzyme activity. When comparing consecutive EMB/DM treatments against previous studies of EMB treatment alone, CYP1A activity levels were similarly diminished, except in muscle. Likewise, FMO activity levels were increased in most of the analyzed tissues, particularly in the muscle, kidney, and intestine. The increases observed for GST were essentially unchanged between consecutive EMB/DM and EMB only treatments. These results indicate that consecutive EMB/DM treatments in rainbow trout induce the expression and activity of FMO and GST enzymes and decrease CYP1A activity. These altered activities of detoxification enzymes could generate imbalances in metabolic processes, synthesis, degradation of hormones and complications associated with drug interactions. It is especially important when analyzing possible effects of consecutive antiparasitic treatments on withholding periods and salmon farming yields.

    KW - Cytochrome P450

    KW - Deltamethrin

    KW - Drug detoxification

    KW - Emamectin benzoate

    KW - Flavin-monooxygenase

    KW - Glutathione S-transferase

    UR - http://www.scopus.com/inward/record.url?scp=84992206096&partnerID=8YFLogxK

    U2 - 10.1016/j.cbpc.2016.10.004

    DO - 10.1016/j.cbpc.2016.10.004

    M3 - Article

    VL - 191

    SP - 129

    EP - 137

    JO - Comparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology

    T2 - Comparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology

    JF - Comparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology

    SN - 0742-8413

    ER -