Dynein regulators are important for ecotropic murine leukemia virus infection

Roger Valle-Tenney, Tatiana Opazo, Jorge Cancino, Stephen P. Goff, Gloria Arriagada

Resultado de la investigación: Research - revisión exhaustivaArticle

  • 2 Citas

Resumen

During the early steps of infection, retroviruses must direct the movement of the viral genome into the nucleus to complete their replication cycle. This process is mediated by cellular proteins that interact first with the reverse transcription complex and later with the preintegration complex (PIC), allowing it to reach and enter the nucleus. For simple retroviruses, such as murine leukemia virus (MLV), the identities of the cellular proteins involved in trafficking of the PIC in infection are unknown. To identify cellular proteins that interact with the MLV PIC, we developed a replication-competent MLV in which the integrase protein was tagged with a FLAG epitope. Using a combination of immunoprecipitation and mass spectrometry, we established that the microtubule motor dynein regulator DCTN2/p50/dynamitin interacts with the MLV preintegration complex early in infection, suggesting a direct interaction between the incoming viral particles and the dynein complex regulators. Further experiments showed that RNA interference (RNAi)-mediated silencing of either DCTN2/p50/dynamitin or another dynein regulator, NudEL, profoundly reduced the efficiency of infection by ecotropic, but not amphotropic, MLV reporters. We propose that the cytoplasmic dynein regulators are a critical component of the host machinery needed for infection by the retroviruses entering the cell via the ecotropic envelope pathway.

IdiomaEnglish
Páginas6896-6905
Número de páginas10
PublicaciónJournal of Virology
Volumen90
Número de edición15
DOI
EstadoPublished - 2016

Huella dactilar

Dyneins
Murine Leukemia Viruses
Virus Diseases
Murine leukemia virus
infection
Proteins
Infection
Retroviridae
proteins
Retroviridae Infections
Dynactin Complex
Cytoplasmic Dyneins
Integrases
Viral Genome
RNA Interference
Immunoprecipitation
Microtubules
Virion
Reverse Transcription
Epitopes

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Microbiology
  • Insect Science

Citar esto

Valle-Tenney, R., Opazo, T., Cancino, J., Goff, S. P., & Arriagada, G. (2016). Dynein regulators are important for ecotropic murine leukemia virus infection. Journal of Virology, 90(15), 6896-6905. DOI: 10.1128/JVI.00863-16
Valle-Tenney, Roger ; Opazo, Tatiana ; Cancino, Jorge ; Goff, Stephen P. ; Arriagada, Gloria. / Dynein regulators are important for ecotropic murine leukemia virus infection. En: Journal of Virology. 2016 ; Vol. 90, N.º 15. pp. 6896-6905
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abstract = "During the early steps of infection, retroviruses must direct the movement of the viral genome into the nucleus to complete their replication cycle. This process is mediated by cellular proteins that interact first with the reverse transcription complex and later with the preintegration complex (PIC), allowing it to reach and enter the nucleus. For simple retroviruses, such as murine leukemia virus (MLV), the identities of the cellular proteins involved in trafficking of the PIC in infection are unknown. To identify cellular proteins that interact with the MLV PIC, we developed a replication-competent MLV in which the integrase protein was tagged with a FLAG epitope. Using a combination of immunoprecipitation and mass spectrometry, we established that the microtubule motor dynein regulator DCTN2/p50/dynamitin interacts with the MLV preintegration complex early in infection, suggesting a direct interaction between the incoming viral particles and the dynein complex regulators. Further experiments showed that RNA interference (RNAi)-mediated silencing of either DCTN2/p50/dynamitin or another dynein regulator, NudEL, profoundly reduced the efficiency of infection by ecotropic, but not amphotropic, MLV reporters. We propose that the cytoplasmic dynein regulators are a critical component of the host machinery needed for infection by the retroviruses entering the cell via the ecotropic envelope pathway.",
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Valle-Tenney, R, Opazo, T, Cancino, J, Goff, SP & Arriagada, G 2016, 'Dynein regulators are important for ecotropic murine leukemia virus infection' Journal of Virology, vol. 90, n.º 15, pp. 6896-6905. DOI: 10.1128/JVI.00863-16

Dynein regulators are important for ecotropic murine leukemia virus infection. / Valle-Tenney, Roger; Opazo, Tatiana; Cancino, Jorge; Goff, Stephen P.; Arriagada, Gloria.

En: Journal of Virology, Vol. 90, N.º 15, 2016, p. 6896-6905.

Resultado de la investigación: Research - revisión exhaustivaArticle

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Valle-Tenney R, Opazo T, Cancino J, Goff SP, Arriagada G. Dynein regulators are important for ecotropic murine leukemia virus infection. Journal of Virology. 2016;90(15):6896-6905. Disponible desde, DOI: 10.1128/JVI.00863-16