Functional evidence of the involvement of the dynein light chain DYNLRB2 in murine leukemia virus infection

Tatiana Opazo, Andrea Garcés, Diego Tapia, Felipe Barraza, Angélica Bravo, Tomás Schwenke, Jorge Cancino, Gloria Arriagada

Resultado de la investigación: Research - revisión exhaustivaArticle

  • 1 Citas

Resumen

How murine leukemia virus (MLV) travels from the cell membrane to the nucleus and the mechanism for nuclear entry of MLV DNA in dividing cells still remain unclear. It seems likely that the MLV preintegration complex (PIC) interacts with cellular proteins to perform these tasks. We recently published that the microtubule motor cytoplasmic dynein complex and its regulator proteins interact with the MLV PIC at early times of infection, suggesting a functional interaction between the incoming viral particles, the dynein complex, and dynein regulators. To better understand the role of the dynein complex in MLV infection, we performed short hairpin RNA (shRNA) screening of the dynein light chains on MLV infection. We found that silencing of a specific light chain of the cytoplasmic dynein complex, DYNLRB2, reduced the efficiency of infection by MLV reporter viruses without affecting HIV-1 infection. Furthermore, the overexpression of DYNLRB2 increased infection by MLV. We conclude that the DYNLRB2 light chain of the cytoplasmic dynein complex is an important and specific piece of the host machinery needed for MLV infection.

IdiomaEnglish
Número de artículoe00129-17
PublicaciónJournal of Virology
Volumen91
Número de edición10
DOI
EstadoPublished - 1 may 2017

Huella dactilar

Dyneins
Murine Leukemia Viruses
Virus Diseases
Murine leukemia virus
infection
Cytoplasmic Dyneins
Infection
Light
Proteins
proteins
Microtubules
Virion
Small Interfering RNA
HIV Infections
HIV-1
Cell Membrane
Viruses
DNA
small interfering RNA
Human immunodeficiency virus 1

Keywords

    ASJC Scopus subject areas

    • Immunology
    • Virology

    Citar esto

    Opazo, T., Garcés, A., Tapia, D., Barraza, F., Bravo, A., Schwenke, T., ... Arriagada, G. (2017). Functional evidence of the involvement of the dynein light chain DYNLRB2 in murine leukemia virus infection. Journal of Virology, 91(10), [e00129-17]. DOI: 10.1128/JVI.00129-17
    Opazo, Tatiana ; Garcés, Andrea ; Tapia, Diego ; Barraza, Felipe ; Bravo, Angélica ; Schwenke, Tomás ; Cancino, Jorge ; Arriagada, Gloria. / Functional evidence of the involvement of the dynein light chain DYNLRB2 in murine leukemia virus infection. En: Journal of Virology. 2017 ; Vol. 91, N.º 10.
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    title = "Functional evidence of the involvement of the dynein light chain DYNLRB2 in murine leukemia virus infection",
    abstract = "How murine leukemia virus (MLV) travels from the cell membrane to the nucleus and the mechanism for nuclear entry of MLV DNA in dividing cells still remain unclear. It seems likely that the MLV preintegration complex (PIC) interacts with cellular proteins to perform these tasks. We recently published that the microtubule motor cytoplasmic dynein complex and its regulator proteins interact with the MLV PIC at early times of infection, suggesting a functional interaction between the incoming viral particles, the dynein complex, and dynein regulators. To better understand the role of the dynein complex in MLV infection, we performed short hairpin RNA (shRNA) screening of the dynein light chains on MLV infection. We found that silencing of a specific light chain of the cytoplasmic dynein complex, DYNLRB2, reduced the efficiency of infection by MLV reporter viruses without affecting HIV-1 infection. Furthermore, the overexpression of DYNLRB2 increased infection by MLV. We conclude that the DYNLRB2 light chain of the cytoplasmic dynein complex is an important and specific piece of the host machinery needed for MLV infection.",
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    Opazo, T, Garcés, A, Tapia, D, Barraza, F, Bravo, A, Schwenke, T, Cancino, J & Arriagada, G 2017, 'Functional evidence of the involvement of the dynein light chain DYNLRB2 in murine leukemia virus infection' Journal of Virology, vol. 91, n.º 10, e00129-17. DOI: 10.1128/JVI.00129-17

    Functional evidence of the involvement of the dynein light chain DYNLRB2 in murine leukemia virus infection. / Opazo, Tatiana; Garcés, Andrea; Tapia, Diego; Barraza, Felipe; Bravo, Angélica; Schwenke, Tomás; Cancino, Jorge; Arriagada, Gloria.

    En: Journal of Virology, Vol. 91, N.º 10, e00129-17, 01.05.2017.

    Resultado de la investigación: Research - revisión exhaustivaArticle

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    AU - Garcés,Andrea

    AU - Tapia,Diego

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    AU - Bravo,Angélica

    AU - Schwenke,Tomás

    AU - Cancino,Jorge

    AU - Arriagada,Gloria

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    N2 - How murine leukemia virus (MLV) travels from the cell membrane to the nucleus and the mechanism for nuclear entry of MLV DNA in dividing cells still remain unclear. It seems likely that the MLV preintegration complex (PIC) interacts with cellular proteins to perform these tasks. We recently published that the microtubule motor cytoplasmic dynein complex and its regulator proteins interact with the MLV PIC at early times of infection, suggesting a functional interaction between the incoming viral particles, the dynein complex, and dynein regulators. To better understand the role of the dynein complex in MLV infection, we performed short hairpin RNA (shRNA) screening of the dynein light chains on MLV infection. We found that silencing of a specific light chain of the cytoplasmic dynein complex, DYNLRB2, reduced the efficiency of infection by MLV reporter viruses without affecting HIV-1 infection. Furthermore, the overexpression of DYNLRB2 increased infection by MLV. We conclude that the DYNLRB2 light chain of the cytoplasmic dynein complex is an important and specific piece of the host machinery needed for MLV infection.

    AB - How murine leukemia virus (MLV) travels from the cell membrane to the nucleus and the mechanism for nuclear entry of MLV DNA in dividing cells still remain unclear. It seems likely that the MLV preintegration complex (PIC) interacts with cellular proteins to perform these tasks. We recently published that the microtubule motor cytoplasmic dynein complex and its regulator proteins interact with the MLV PIC at early times of infection, suggesting a functional interaction between the incoming viral particles, the dynein complex, and dynein regulators. To better understand the role of the dynein complex in MLV infection, we performed short hairpin RNA (shRNA) screening of the dynein light chains on MLV infection. We found that silencing of a specific light chain of the cytoplasmic dynein complex, DYNLRB2, reduced the efficiency of infection by MLV reporter viruses without affecting HIV-1 infection. Furthermore, the overexpression of DYNLRB2 increased infection by MLV. We conclude that the DYNLRB2 light chain of the cytoplasmic dynein complex is an important and specific piece of the host machinery needed for MLV infection.

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    Opazo T, Garcés A, Tapia D, Barraza F, Bravo A, Schwenke T y otros. Functional evidence of the involvement of the dynein light chain DYNLRB2 in murine leukemia virus infection. Journal of Virology. 2017 may 1;91(10). e00129-17. Disponible desde, DOI: 10.1128/JVI.00129-17