Peptide multifunctionalized gold nanorods decrease toxicity of β-amyloid peptide in a Caenorhabditis elegans model of Alzheimer's disease

Francisco Morales-Zavala, Hector Arriagada, Natalia Hassan, Carolina Velasco, Ana Riveros, Alejandra R. Álvarez, Alicia N. Minniti, Ximena Rojas-Silva, Luis L. Muñoz, Rodrigo Vasquez, Katherine Rodriguez, Macarena Sanchez-Navarro, Ernest Giralt, Eyleen Araya, Rebeca Aldunate, Marcelo J. Kogan

Resultado de la investigación: Research - revisión exhaustivaArticle

Resumen

The properties of nanometric materials make nanotechnology a promising platform for tackling problems of contemporary medicine. In this work, gold nanorods were synthetized and stabilized with polyethylene glycols and modified with two kinds of peptides. The D1 peptide that recognizes toxic aggregates of Aβ, a peptide involved in Alzheimer's disease (AD); and the Angiopep 2 that can be used to deliver nanorods to the mammalian central nervous system. The nanoconjugates were characterized using absorption spectrophotometry, dynamic light scattering, and transmission electron microscopy, among other techniques. We determined that the nanoconjugate does not affect neuronal viability; it penetrates the cells, and decreases aggregation of Aβ peptide in vitro. We also showed that when we apply our nanosystem to a Caenorhabditis elegans AD model, the toxicity of aggregated Aβ peptide is decreased. This work may contribute to the development of therapies for AD based on metallic nanoparticles.

IdiomaEnglish
Páginas2341-2350
Número de páginas10
PublicaciónNanomedicine: Nanotechnology, Biology, and Medicine
Volumen13
Número de edición7
DOI
EstadoPublished - 1 oct 2017
Publicado de forma externa

Huella dactilar

Nanotubes
Amyloid beta-Peptides
Caenorhabditis elegans
Gold
Alzheimer Disease
Peptides
Nanorods
Toxicity
Amyloid
Nanoconjugates
Metal Nanoparticles
Cell Aggregation
Nanotechnology
Poisons
Spectrophotometry
Transmission Electron Microscopy
Central Nervous System
Medicine
Therapeutics
In Vitro Techniques

Keywords

    ASJC Scopus subject areas

    • Medicine (miscellaneous)
    • Bioengineering
    • Biomedical Engineering
    • Molecular Medicine
    • Materials Science(all)
    • Pharmaceutical Science

    Citar esto

    Morales-Zavala, Francisco ; Arriagada, Hector ; Hassan, Natalia ; Velasco, Carolina ; Riveros, Ana ; Álvarez, Alejandra R. ; Minniti, Alicia N. ; Rojas-Silva, Ximena ; Muñoz, Luis L. ; Vasquez, Rodrigo ; Rodriguez, Katherine ; Sanchez-Navarro, Macarena ; Giralt, Ernest ; Araya, Eyleen ; Aldunate, Rebeca ; Kogan, Marcelo J./ Peptide multifunctionalized gold nanorods decrease toxicity of β-amyloid peptide in a Caenorhabditis elegans model of Alzheimer's disease. En: Nanomedicine: Nanotechnology, Biology, and Medicine. 2017 ; Vol. 13, N.º 7. pp. 2341-2350
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    abstract = "The properties of nanometric materials make nanotechnology a promising platform for tackling problems of contemporary medicine. In this work, gold nanorods were synthetized and stabilized with polyethylene glycols and modified with two kinds of peptides. The D1 peptide that recognizes toxic aggregates of Aβ, a peptide involved in Alzheimer's disease (AD); and the Angiopep 2 that can be used to deliver nanorods to the mammalian central nervous system. The nanoconjugates were characterized using absorption spectrophotometry, dynamic light scattering, and transmission electron microscopy, among other techniques. We determined that the nanoconjugate does not affect neuronal viability; it penetrates the cells, and decreases aggregation of Aβ peptide in vitro. We also showed that when we apply our nanosystem to a Caenorhabditis elegans AD model, the toxicity of aggregated Aβ peptide is decreased. This work may contribute to the development of therapies for AD based on metallic nanoparticles.",
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    author = "Francisco Morales-Zavala and Hector Arriagada and Natalia Hassan and Carolina Velasco and Ana Riveros and Álvarez, {Alejandra R.} and Minniti, {Alicia N.} and Ximena Rojas-Silva and Muñoz, {Luis L.} and Rodrigo Vasquez and Katherine Rodriguez and Macarena Sanchez-Navarro and Ernest Giralt and Eyleen Araya and Rebeca Aldunate and Kogan, {Marcelo J.}",
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    Morales-Zavala, F, Arriagada, H, Hassan, N, Velasco, C, Riveros, A, Álvarez, AR, Minniti, AN, Rojas-Silva, X, Muñoz, LL, Vasquez, R, Rodriguez, K, Sanchez-Navarro, M, Giralt, E, Araya, E, Aldunate, R & Kogan, MJ 2017, 'Peptide multifunctionalized gold nanorods decrease toxicity of β-amyloid peptide in a Caenorhabditis elegans model of Alzheimer's disease' Nanomedicine: Nanotechnology, Biology, and Medicine, vol. 13, n.º 7, pp. 2341-2350. DOI: 10.1016/j.nano.2017.06.013

    Peptide multifunctionalized gold nanorods decrease toxicity of β-amyloid peptide in a Caenorhabditis elegans model of Alzheimer's disease. / Morales-Zavala, Francisco; Arriagada, Hector; Hassan, Natalia; Velasco, Carolina; Riveros, Ana; Álvarez, Alejandra R.; Minniti, Alicia N.; Rojas-Silva, Ximena; Muñoz, Luis L.; Vasquez, Rodrigo; Rodriguez, Katherine; Sanchez-Navarro, Macarena; Giralt, Ernest; Araya, Eyleen; Aldunate, Rebeca; Kogan, Marcelo J.

    En: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 13, N.º 7, 01.10.2017, p. 2341-2350.

    Resultado de la investigación: Research - revisión exhaustivaArticle

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    AU - Hassan,Natalia

    AU - Velasco,Carolina

    AU - Riveros,Ana

    AU - Álvarez,Alejandra R.

    AU - Minniti,Alicia N.

    AU - Rojas-Silva,Ximena

    AU - Muñoz,Luis L.

    AU - Vasquez,Rodrigo

    AU - Rodriguez,Katherine

    AU - Sanchez-Navarro,Macarena

    AU - Giralt,Ernest

    AU - Araya,Eyleen

    AU - Aldunate,Rebeca

    AU - Kogan,Marcelo J.

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