The root hair specific SYP123 regulates the localization of cell wall components and contributes to rizhobacterial priming of induced systemic resistance

Cecilia Rodriguez-Furlán, Hernán Salinas-Grenet, Omar Sandoval, Camilo Recabarren, Paulina Arraño-Salinas, Sylvana Soto-Alvear, Ariel Orellana, Francisca Blanco-Herrera

Resultado de la investigación: Contribución a la publicaciónArticle

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Resumen

Root hairs are important for nutrient and water uptake and are also critically involved the interaction with soil inhabiting microbiota. Root hairs are tubular-shaped outgrowths that emerge from trichoblasts. This polarized elongation is maintained and regulated by a robust mechanism involving the endomembrane secretory and endocytic system. Members of the syntaxin family of SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) in plants (SYP), have been implicated in regulation of the fusion of vesicles with the target membranes in both exocytic and endocytic pathways. One member of this family, SYP123, is expressed specifically in the root hairs and accumulated in the growing tip region. This study shows evidence of the SYP123 role in polarized trafficking using knockout insertional mutant plants. We were able to observe defects in the deposition of cell wall proline rich protein PRP3 and cell wall polysaccharides. In a complementary strategy, similar results were obtained using a plant expressing a dominant negative soluble version of SYP123 (SP2 fragment) lacking the transmembrane domain. The evidence presented indicates that SYP123 is also regulating PRP3 protein distribution by recycling by endocytosis. We also present evidence that indicates that SYP123 is necessary for the response of roots to plant growth promoting rhizobacterium (PGPR) in order to trigger trigger induced systemic response (ISR). Plants with a defective SYP123 function were unable to mount a systemic acquired resistance in response to bacterial pathogen infection and ISR upon interaction with rhizobacteria. These results indicated that SYP123 was involved in the polarized localization of protein and polysaccharides in growing root hairs and that this activity also contributed to the establishment of effective plant defense responses. Root hairs represent very plastic structures were many biotic and abiotic factors can affect the number, anatomy and physiology of root hairs. Here, we presented evidence that indicates that interactions with soil PGPR could be closely regulated by signaling involving secretory and/or endocytic trafficking at the root hair tip as a quick way to response to changing environmental conditions.

Idioma originalEnglish
Número de artículo1081
PublicaciónFrontiers in Plant Science
Volumen7
Número de ediciónJULY2016
Identificadores de objetos digitales
EstadoPublished - 26 jul 2016

Huella dactilar

root hairs
proteins
plant growth-promoting rhizobacteria
polysaccharides
cell walls
systemic acquired resistance
rhizosphere bacteria
endocytosis
cell wall components
soil microorganisms
water uptake
nutrient uptake
recycling
proline
physiology
plastics
mutants
receptors
environmental factors
pathogens

Keywords

    ASJC Scopus subject areas

    • Plant Science

    Citar esto

    Rodriguez-Furlán, Cecilia; Salinas-Grenet, Hernán; Sandoval, Omar; Recabarren, Camilo; Arraño-Salinas, Paulina; Soto-Alvear, Sylvana; Orellana, Ariel; Blanco-Herrera, Francisca / The root hair specific SYP123 regulates the localization of cell wall components and contributes to rizhobacterial priming of induced systemic resistance.

    En: Frontiers in Plant Science, Vol. 7, N.º JULY2016, 1081, 26.07.2016.

    Resultado de la investigación: Contribución a la publicaciónArticle

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    abstract = "Root hairs are important for nutrient and water uptake and are also critically involved the interaction with soil inhabiting microbiota. Root hairs are tubular-shaped outgrowths that emerge from trichoblasts. This polarized elongation is maintained and regulated by a robust mechanism involving the endomembrane secretory and endocytic system. Members of the syntaxin family of SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) in plants (SYP), have been implicated in regulation of the fusion of vesicles with the target membranes in both exocytic and endocytic pathways. One member of this family, SYP123, is expressed specifically in the root hairs and accumulated in the growing tip region. This study shows evidence of the SYP123 role in polarized trafficking using knockout insertional mutant plants. We were able to observe defects in the deposition of cell wall proline rich protein PRP3 and cell wall polysaccharides. In a complementary strategy, similar results were obtained using a plant expressing a dominant negative soluble version of SYP123 (SP2 fragment) lacking the transmembrane domain. The evidence presented indicates that SYP123 is also regulating PRP3 protein distribution by recycling by endocytosis. We also present evidence that indicates that SYP123 is necessary for the response of roots to plant growth promoting rhizobacterium (PGPR) in order to trigger trigger induced systemic response (ISR). Plants with a defective SYP123 function were unable to mount a systemic acquired resistance in response to bacterial pathogen infection and ISR upon interaction with rhizobacteria. These results indicated that SYP123 was involved in the polarized localization of protein and polysaccharides in growing root hairs and that this activity also contributed to the establishment of effective plant defense responses. Root hairs represent very plastic structures were many biotic and abiotic factors can affect the number, anatomy and physiology of root hairs. Here, we presented evidence that indicates that interactions with soil PGPR could be closely regulated by signaling involving secretory and/or endocytic trafficking at the root hair tip as a quick way to response to changing environmental conditions.",
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    author = "Cecilia Rodriguez-Furlán and Hernán Salinas-Grenet and Omar Sandoval and Camilo Recabarren and Paulina Arraño-Salinas and Sylvana Soto-Alvear and Ariel Orellana and Francisca Blanco-Herrera",
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    The root hair specific SYP123 regulates the localization of cell wall components and contributes to rizhobacterial priming of induced systemic resistance. / Rodriguez-Furlán, Cecilia; Salinas-Grenet, Hernán; Sandoval, Omar; Recabarren, Camilo; Arraño-Salinas, Paulina; Soto-Alvear, Sylvana; Orellana, Ariel; Blanco-Herrera, Francisca.

    En: Frontiers in Plant Science, Vol. 7, N.º JULY2016, 1081, 26.07.2016.

    Resultado de la investigación: Contribución a la publicaciónArticle

    TY - JOUR

    T1 - The root hair specific SYP123 regulates the localization of cell wall components and contributes to rizhobacterial priming of induced systemic resistance

    AU - Rodriguez-Furlán,Cecilia

    AU - Salinas-Grenet,Hernán

    AU - Sandoval,Omar

    AU - Recabarren,Camilo

    AU - Arraño-Salinas,Paulina

    AU - Soto-Alvear,Sylvana

    AU - Orellana,Ariel

    AU - Blanco-Herrera,Francisca

    PY - 2016/7/26

    Y1 - 2016/7/26

    N2 - Root hairs are important for nutrient and water uptake and are also critically involved the interaction with soil inhabiting microbiota. Root hairs are tubular-shaped outgrowths that emerge from trichoblasts. This polarized elongation is maintained and regulated by a robust mechanism involving the endomembrane secretory and endocytic system. Members of the syntaxin family of SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) in plants (SYP), have been implicated in regulation of the fusion of vesicles with the target membranes in both exocytic and endocytic pathways. One member of this family, SYP123, is expressed specifically in the root hairs and accumulated in the growing tip region. This study shows evidence of the SYP123 role in polarized trafficking using knockout insertional mutant plants. We were able to observe defects in the deposition of cell wall proline rich protein PRP3 and cell wall polysaccharides. In a complementary strategy, similar results were obtained using a plant expressing a dominant negative soluble version of SYP123 (SP2 fragment) lacking the transmembrane domain. The evidence presented indicates that SYP123 is also regulating PRP3 protein distribution by recycling by endocytosis. We also present evidence that indicates that SYP123 is necessary for the response of roots to plant growth promoting rhizobacterium (PGPR) in order to trigger trigger induced systemic response (ISR). Plants with a defective SYP123 function were unable to mount a systemic acquired resistance in response to bacterial pathogen infection and ISR upon interaction with rhizobacteria. These results indicated that SYP123 was involved in the polarized localization of protein and polysaccharides in growing root hairs and that this activity also contributed to the establishment of effective plant defense responses. Root hairs represent very plastic structures were many biotic and abiotic factors can affect the number, anatomy and physiology of root hairs. Here, we presented evidence that indicates that interactions with soil PGPR could be closely regulated by signaling involving secretory and/or endocytic trafficking at the root hair tip as a quick way to response to changing environmental conditions.

    AB - Root hairs are important for nutrient and water uptake and are also critically involved the interaction with soil inhabiting microbiota. Root hairs are tubular-shaped outgrowths that emerge from trichoblasts. This polarized elongation is maintained and regulated by a robust mechanism involving the endomembrane secretory and endocytic system. Members of the syntaxin family of SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) in plants (SYP), have been implicated in regulation of the fusion of vesicles with the target membranes in both exocytic and endocytic pathways. One member of this family, SYP123, is expressed specifically in the root hairs and accumulated in the growing tip region. This study shows evidence of the SYP123 role in polarized trafficking using knockout insertional mutant plants. We were able to observe defects in the deposition of cell wall proline rich protein PRP3 and cell wall polysaccharides. In a complementary strategy, similar results were obtained using a plant expressing a dominant negative soluble version of SYP123 (SP2 fragment) lacking the transmembrane domain. The evidence presented indicates that SYP123 is also regulating PRP3 protein distribution by recycling by endocytosis. We also present evidence that indicates that SYP123 is necessary for the response of roots to plant growth promoting rhizobacterium (PGPR) in order to trigger trigger induced systemic response (ISR). Plants with a defective SYP123 function were unable to mount a systemic acquired resistance in response to bacterial pathogen infection and ISR upon interaction with rhizobacteria. These results indicated that SYP123 was involved in the polarized localization of protein and polysaccharides in growing root hairs and that this activity also contributed to the establishment of effective plant defense responses. Root hairs represent very plastic structures were many biotic and abiotic factors can affect the number, anatomy and physiology of root hairs. Here, we presented evidence that indicates that interactions with soil PGPR could be closely regulated by signaling involving secretory and/or endocytic trafficking at the root hair tip as a quick way to response to changing environmental conditions.

    KW - Cell wall

    KW - Induced systemic resistance

    KW - Plant growth promoting rhizobacterium

    KW - PRP3

    KW - Rhizobacteria

    KW - Syntaxin

    KW - Systemic acquired resistance

    KW - Trafficking

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    DO - 10.3389/fpls.2016.01081

    M3 - Article

    VL - 7

    JO - Frontiers in Plant Science

    T2 - Frontiers in Plant Science

    JF - Frontiers in Plant Science

    SN - 1664-462X

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    Rodriguez-Furlán C, Salinas-Grenet H, Sandoval O, Recabarren C, Arraño-Salinas P, Soto-Alvear S y otros. The root hair specific SYP123 regulates the localization of cell wall components and contributes to rizhobacterial priming of induced systemic resistance. Frontiers in Plant Science. 2016 jul 26;7(JULY2016). 1081. Disponible desde, DOI: 10.3389/fpls.2016.01081