The UDP-glucose: Glycoprotein glucosyltransferase (UGGT), a key enzyme in ER quality control, plays a significant role in plant growth as well as biotic and abiotic stress in Arabidopsis thaliana

Francisca Blanco-Herrera, Adrián A. Moreno, Rodrigo Tapia, Francisca Reyes, Macarena Araya, Cecilia D'Alessio, Armando Parodi, Ariel Orellana

Resultado de la investigación: Contribución a la publicaciónArticle

  • 14 Citas

Resumen

Background: UDP-glucose: glycoprotein glucosyltransferase (UGGT) is a key player in the quality control mechanism (ER-QC) that newly synthesized glycoproteins undergo in the ER. It has been shown that the UGGT Arabidopsis orthologue is involved in ER-QC; however, its role in plant physiology remains unclear. Results: Here, we show that two mutant alleles in the At1g71220 locus have none or reduced UGGT activity. In wild type plants, the AtUGGT transcript levels increased upon activation of the unfolded protein response (UPR). Interestingly, mutants in AtUGGT exhibited an endogenous up-regulation of genes that are UPR targets. In addition, mutants in AtUGGT showed a 30 % reduction in the incorporation of UDP-Glucose into the ER suggesting that this enzyme drives the uptake of this substrate for the CNX/CRT cycle. Plants deficient in UGGT exhibited a delayed growth rate of the primary root and rosette as well as an alteration in the number of leaves. These mutants are more sensitive to pathogen attack as well as heat, salt, and UPR-inducing stressors. Additionally, the plants showed impairment in the establishment of systemic acquired resistance (SAR). Conclusions: These results show that a lack of UGGT activity alters plant vegetative development and impairs the response to several abiotic and biotic stresses. Moreover, our results uncover an unexpected role of UGGT in the incorporation of UDP-Glucose into the ER lumen in Arabidopsis thaliana.

Idioma originalEnglish
Número de artículo127
PublicaciónBMC Plant Biology
Volumen15
Número de edición1
Identificadores de objetos digitales
EstadoPublished - 28 may 2015

Huella dactilar

glucose
glycoproteins
glucosyltransferases
mutants
unfolded protein response
biotic stress
abiotic stress
quality control
Arabidopsis thaliana
enzymes
systemic acquired resistance
plant physiology
Arabidopsis
plant growth
salts
alleles
uptake mechanisms
heat
loci
pathogens

Keywords

    ASJC Scopus subject areas

    • Plant Science

    Citar esto

    Blanco-Herrera, Francisca; Moreno, Adrián A.; Tapia, Rodrigo; Reyes, Francisca; Araya, Macarena; D'Alessio, Cecilia; Parodi, Armando; Orellana, Ariel / The UDP-glucose : Glycoprotein glucosyltransferase (UGGT), a key enzyme in ER quality control, plays a significant role in plant growth as well as biotic and abiotic stress in Arabidopsis thaliana.

    En: BMC Plant Biology, Vol. 15, N.º 1, 127, 28.05.2015.

    Resultado de la investigación: Contribución a la publicaciónArticle

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    title = "The UDP-glucose: Glycoprotein glucosyltransferase (UGGT), a key enzyme in ER quality control, plays a significant role in plant growth as well as biotic and abiotic stress in Arabidopsis thaliana",
    abstract = "Background: UDP-glucose: glycoprotein glucosyltransferase (UGGT) is a key player in the quality control mechanism (ER-QC) that newly synthesized glycoproteins undergo in the ER. It has been shown that the UGGT Arabidopsis orthologue is involved in ER-QC; however, its role in plant physiology remains unclear. Results: Here, we show that two mutant alleles in the At1g71220 locus have none or reduced UGGT activity. In wild type plants, the AtUGGT transcript levels increased upon activation of the unfolded protein response (UPR). Interestingly, mutants in AtUGGT exhibited an endogenous up-regulation of genes that are UPR targets. In addition, mutants in AtUGGT showed a 30 % reduction in the incorporation of UDP-Glucose into the ER suggesting that this enzyme drives the uptake of this substrate for the CNX/CRT cycle. Plants deficient in UGGT exhibited a delayed growth rate of the primary root and rosette as well as an alteration in the number of leaves. These mutants are more sensitive to pathogen attack as well as heat, salt, and UPR-inducing stressors. Additionally, the plants showed impairment in the establishment of systemic acquired resistance (SAR). Conclusions: These results show that a lack of UGGT activity alters plant vegetative development and impairs the response to several abiotic and biotic stresses. Moreover, our results uncover an unexpected role of UGGT in the incorporation of UDP-Glucose into the ER lumen in Arabidopsis thaliana.",
    keywords = "Abiotic stress, Biotic stress, Endoplasmic reticulum, UGGT",
    author = "Francisca Blanco-Herrera and Moreno, {Adrián A.} and Rodrigo Tapia and Francisca Reyes and Macarena Araya and Cecilia D'Alessio and Armando Parodi and Ariel Orellana",
    year = "2015",
    month = "5",
    doi = "10.1186/s12870-015-0525-2",
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    journal = "BMC Plant Biology",
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    The UDP-glucose : Glycoprotein glucosyltransferase (UGGT), a key enzyme in ER quality control, plays a significant role in plant growth as well as biotic and abiotic stress in Arabidopsis thaliana. / Blanco-Herrera, Francisca; Moreno, Adrián A.; Tapia, Rodrigo; Reyes, Francisca; Araya, Macarena; D'Alessio, Cecilia; Parodi, Armando; Orellana, Ariel.

    En: BMC Plant Biology, Vol. 15, N.º 1, 127, 28.05.2015.

    Resultado de la investigación: Contribución a la publicaciónArticle

    TY - JOUR

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    T2 - BMC Plant Biology

    AU - Blanco-Herrera,Francisca

    AU - Moreno,Adrián A.

    AU - Tapia,Rodrigo

    AU - Reyes,Francisca

    AU - Araya,Macarena

    AU - D'Alessio,Cecilia

    AU - Parodi,Armando

    AU - Orellana,Ariel

    PY - 2015/5/28

    Y1 - 2015/5/28

    N2 - Background: UDP-glucose: glycoprotein glucosyltransferase (UGGT) is a key player in the quality control mechanism (ER-QC) that newly synthesized glycoproteins undergo in the ER. It has been shown that the UGGT Arabidopsis orthologue is involved in ER-QC; however, its role in plant physiology remains unclear. Results: Here, we show that two mutant alleles in the At1g71220 locus have none or reduced UGGT activity. In wild type plants, the AtUGGT transcript levels increased upon activation of the unfolded protein response (UPR). Interestingly, mutants in AtUGGT exhibited an endogenous up-regulation of genes that are UPR targets. In addition, mutants in AtUGGT showed a 30 % reduction in the incorporation of UDP-Glucose into the ER suggesting that this enzyme drives the uptake of this substrate for the CNX/CRT cycle. Plants deficient in UGGT exhibited a delayed growth rate of the primary root and rosette as well as an alteration in the number of leaves. These mutants are more sensitive to pathogen attack as well as heat, salt, and UPR-inducing stressors. Additionally, the plants showed impairment in the establishment of systemic acquired resistance (SAR). Conclusions: These results show that a lack of UGGT activity alters plant vegetative development and impairs the response to several abiotic and biotic stresses. Moreover, our results uncover an unexpected role of UGGT in the incorporation of UDP-Glucose into the ER lumen in Arabidopsis thaliana.

    AB - Background: UDP-glucose: glycoprotein glucosyltransferase (UGGT) is a key player in the quality control mechanism (ER-QC) that newly synthesized glycoproteins undergo in the ER. It has been shown that the UGGT Arabidopsis orthologue is involved in ER-QC; however, its role in plant physiology remains unclear. Results: Here, we show that two mutant alleles in the At1g71220 locus have none or reduced UGGT activity. In wild type plants, the AtUGGT transcript levels increased upon activation of the unfolded protein response (UPR). Interestingly, mutants in AtUGGT exhibited an endogenous up-regulation of genes that are UPR targets. In addition, mutants in AtUGGT showed a 30 % reduction in the incorporation of UDP-Glucose into the ER suggesting that this enzyme drives the uptake of this substrate for the CNX/CRT cycle. Plants deficient in UGGT exhibited a delayed growth rate of the primary root and rosette as well as an alteration in the number of leaves. These mutants are more sensitive to pathogen attack as well as heat, salt, and UPR-inducing stressors. Additionally, the plants showed impairment in the establishment of systemic acquired resistance (SAR). Conclusions: These results show that a lack of UGGT activity alters plant vegetative development and impairs the response to several abiotic and biotic stresses. Moreover, our results uncover an unexpected role of UGGT in the incorporation of UDP-Glucose into the ER lumen in Arabidopsis thaliana.

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    KW - Biotic stress

    KW - Endoplasmic reticulum

    KW - UGGT

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